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chick80
Moderatore
Cittā: Edinburgh
11491 Messaggi |
 Inserito il - 02 febbraio 2007 : 00:27:55
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Se fai una ricerca in Pubmed per "mitochondria cardiomyocyte" puoi trovare un sacco di articoli e andare a spulciare fra i loro materiali e metodi! :D
Ad es:
Cardiac mitochondrial damage and biogenesis in a chronic model of type 1 diabetes - Am J Physiol Endocrinol Metab 2004
Ti incollo anche il loro metodo:
Fresh heart was immediately washed with prechilled isolation buffer containing 220 mM mannitol, 70 mM sucrose, and 5 mM MOPS, pH 7.4. The heart tissues were then minced and homogenized by a motor-driven Teflon pestle in the isolation buffer. The homogenate was centrifuged at 500 g for 10 min at 4°C, and supernatant was collected by passing it through cheesecloth. The filtrate was centrifuged at 5,000 g for 10 min at 4°C. The pellet was washed again with isolation buffer and gently resuspended in respiration buffer (225 mM mannitol, 70 mM sucrose, 10 mM KH2PO4, and 1 mM EGTA, pH 7.2). Protein concentration of the mitochondria preparation was determined by the Lowry method. |
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Isolamento mitocondri di miociti
Didattica
